Thermo Scientific Evolution 300 UV-Vis Spectrophotometer
For over 60 years, customers in world-renowned research institutions, highly regulated QC laboratories and teaching
institutions have relied on Thermo Scientific™ UV-Visible (UV-Vis) spectrophotometers. The Thermo Scientific™ Evolution™ 300 UV-Visible spectrophotometer is founded on the successful legacy of the Pye Unicam, Bausch & Lomb™, Phillips Scientific, and Thermo Scientific™ SPECTRONIC™ products. Many customers today say they made their first absorbance measurement on a Thermo Scientific SPECTRONIC 20 spectrophotometer, a product introduced to the market in 1953. The Evolution 300 continues in this tradition by delivering the high level of performance, accuracy and precision you need for your UV-Vis applications.
Specifications
| Optical Design | Modified Ebert Double beam with sample and reference cuvette/accessory positions |
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| Spectral Bandwidths | Variable 0.5, 1.0, 1.5, 2.0, 4.0 nm | |
| Light Source | Xenon Flash Lamp Typical Lifetime: >5 years. Longer if not using live signal. Warranty Period: 3 year source replacement warranty |
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| Detector | Dual Matched Silicon Photodiodes | |
| Grating | Holographic, 1200 lines/mm, blazed at 240 nm | |
| Beam Separation | 210 mm | |
| Scan Ordinate Modes | Absorbance, % Transmittance, % Reflectance, Concentration, 1st–4th Derivative |
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| Wavelength | Range | 190–1100 nm |
| Accuracy | ±0.20 nm (546.11 nm Hg emission line), ±0.30 nm 190–900 nm | |
| Repeatability | Standard deviation of 10 measurements <0.05 nm | |
| Scanning Speeds | 3800, 2400, 1200, 600, 240, 120, 60, 30, 10, 5, 1 nm/min Intelliscan |
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| Data Interval | 10, 5, 2, 1, 0.5, 0.2, 0.1, 0.05 nm | |
| Photometric | Range | >4 A |
| Display | ±6 A | |
| Readout | Absorbance, % Transmission, % Reflectance, Concentration | |
| Accuracy – Instrument1 | 1A: ±0.004 A 2A: ±0.004 A 3A: ±0.006 A |
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| Accuracy – Including Standard Tolerance2 | 1A: ±0.008 A 2A: ±0.010 A 3A: ±0.018 A |
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| Noise | 0A: <0.00018 A 1A: <0.00022 A 2A: <0.00050 A 500 nm, 2.0 nm SBW, RMS |
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| Repeatability | 1A: ±0.0025 A | |
| Drift | <0.0005 Abs/hour, 500 nm, 2.0 nm SBW, 2 hr warm-up | |
| Stray Light | 198 nm: 2.9 A KCl 220 nm: 4.2 A NaI 340 nm: 4.3 A NaNO2 |
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| Baseline Flatness | ±0.0015 A (200–800 nm) 2.0 nm SBW, smoothed |
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| Dimensions (W × D × H) | 61 × 53 × 38 cm | |
| Weight | 22 kg | |
| Electrical Supply | 100–240 V, 50–60 Hz | |
| Pharmacopoeia Compliance Testing | ||
| Resolution (Toluene in Hexane) | Peak/Trough Ratio >2.0 | |
| Photometric Accuracy (60mg/L K2Cr2O7) | <±0.01 A relative to calibrated sealed standard | |
| Stray Light | <0.13 %T at 198 nm with KCl per EP 220 nm: <0.01%T NaI (behaves identically to KI for USP) |
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| Wavelength Accuracy | ±0.20 nm (546.11 nm Hg emission line), ±0.30 nm 190–900 nm | |
| Wavelength Repeatability | Peak separation of repetitive scanning of Hg line source <0.10 nm | |