StepOne & StepOnePlus Real-Time PCR Systems

StepOne & StepOnePlus Real-Time PCR Systems

The Applied Biosystems StepOne and StepOnePlus Real-Time PCR Systems offer intuitive software, Fast (<40 min) and standard (<2 hr) runs, and are configured for PC-free or networked operation. These factory-calibrated systems make it easy to step up to high-performance qPCR. And now these systems support high resolution melt (HRM) software.

Real-time PCRapplication

  • SNP genotyping
  • Translocation analysis
  • Gene expression profi ling
  • Gene detection
  • MicroRNA expression
  • Viral load analysis
  • Methylation
  • Mutation scanning
StepOne System

  • The StepOne System is a remarkably simple real-time PCR system that enables precise quantitative real-time PCR results.

    • Easy to use
    • High-quality qPCR results
    • Cost-effective, 3-color, 48-well instrument
    • Designed with a user-friendly, powerful interface for researchers new to real-time PCR
StepOnePlus System

  • The StepOnePlus System includes additional performance features supporting the full range of  Applied Biosystems TaqMan® Assays, while providing ease of use and a small footprint.

    • Easy to use
    • High-quality qPCR results
    • 4-color, 96-well PCR instrument
    • Innovative Applied Biosystems VeriFlex Block for convenient PCR optimization

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QuantStudio real-time PCR and digital PCR systems
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Real-time PCR solutions brochure
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QuantStudio 12K Flex Real-Time PCR System

QuantStudio 12K Flex Real-Time PCR System

Combining flexible throughput capabilities with a streamlined workflow, the Applied Biosystems™ QuantStudio™ 12K Flex system takes you from targeted discovery through confirmation and screening, all on a single platform

Maximum Throughput

Maximize throughput with minimal resources.
Increase your throughput by as much as 32-fold by running 4 Applied Biosystems™ OpenArray™ plates in a single run on the Applied Biosystems™ QuantStudio™ 12K Flex system. It’s the equivalent of 32 traditional 384-well qPCR plates or 12,000 data points.

Push your throughput even more.
When equipped with the OpenArray block, a single 9700 thermal cycler and the QuantStudio 12K Flex Applied Biosystems™ OpenArray™ AccuFill™ System, the QuantStudio 12K Flex system can produce up to 110,000 data points or more in an eight-hour day. 

Reduce start up time and hands on time.
Start a 12,000 data point experiment typically in 20 minutes or less.

Flexibility & Scalability

Includes five interchangeable blocks

The QuantStudio 12K Flex blocks accomodate 96-well, FAST 96-well, 384-well, TaqMan array cards, and OpenArray plates, for effortless scaling from 1 to 12,000 data points in a single run.

Compatible with a full range of TaqMan assay

The QuantStudio 12K Flex System is optimized to enable clear, clean data for all TaqMan assays that might be used across a broad range of applications

Provides a seamless switch from qPCR to digital PC

Increase the precision and sensitivity of your nucleic acid experiment by switching from qPCR to digital PCR mode using QuantStudio Digital PCR kits and DigitalSuite™ Software with the QuantStudio OpenArray block.

Real-Time PCR Application

  • Gene expression
  • miRNA profiling
  • SNP genotyping
  • Copy number variation
  • Protein thermal shift
  • High resolution melt
  • Pathogen detection
  • Pharmacogenomics
  • Growing menu of qualified solutions

Product Information PDF

QuantStudio real-time PCR and digital PCR systems
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Real-time PCR solutions brochure
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QuantStudio 7 Flex Real-Time PCR System

QuantStudio 7 Flex Real-Time PCR System

With an upgrade path to the QuantStudio 7 Flex Real-Time System, the QuantStudio 6 system is a great choice if you anticipate your needs will change in the future. The QuantStudio 7 system allows you to run hundreds of real-time PCR reactions using TaqMan Array Microfluidic Cards for maximum throughput in an automated environment.

Key features

Accomplish more, faster
Run hundreds of real-time PCR reactions effortlessly using TaqMan Array Microfluidic Cards integrated with the Twister robot. The QuantStudio 7 Flex system can maximize your automated throughput capabilities.

Gain broad application versatility
Optimized protocols and reagents have been combined with intuitive software to adapt to the widest range of applications.

Achieve results you can trust
Improved well-to-well and instrument-to-instrument data accuracy are achieved with the OptiFlex System, which features six decoupled excitation and emission filter channels with 21 filter combinations for maximum multiplexing and chemistry flexibility.

Product Information PDF

QuantStudio real-time PCR and digital PCR systems
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Real-time PCR solutions brochure
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QuantStudio 7 Pro Real-Time PCR Systems

QuantStudio 6 and 7 Pro Real-Time PCR Systems

The Applied Biosystems QuantStudio 6 and 7 Pro real-time PCR systems are designed to offer high quality and excellent reliability with features to deliver a smart workflow experience that brings lab-changing qPCR innovations to your research. These systems feature an interactive 12.1-in touchscreen interface, intuitive software, pre-optimized protocol templates, and access to your data anytime, anywhere with our Connect cloud-based platform. Additional smart features are included to enable a personalized experience that improves efficiency and helps streamline your workflow.

  • Smart features—hands-free functionality includes facial authentication login, voice commands, and RFID reader
  • Manual error reduction—compatible with TaqMan Array Plates with RFID
  • Interchangeable blocks—flexibility to change block formats between 96- (0.1 and 0.2 mL) and 384-well, and TaqMan Array Cards* via simply pushing a button or using a voice command
  • Connect—access, analyze, and share data anytime, anywhere with Connect, our cloud-based platform for real-time information access, secure data storage, and online results sharing
  • Remote access—monitor experiments and access results remotely using the Instrument Connect app
  • Small footprint—minimal bench space required: only 54.7 x 33.8 x 52.5 cm (H x W x D) and 38 kg (wt)
  • Multiplexing—5 or 6 optical channels (21 filter combinations for QuantStudio 7 Pro system)
  • Automation compatible—increase walkaway time and productivity by adding automatic loading of one or two QuantStudio 7 Pro systems with the Orbitor RS2 Microplate Mover
  • Results you can trust—10 logarithmic units of dynamic range to detect differences as small as 1.5-fold
  • Easy access to help—embedded video help from the instrument
  • Peace of mind—superior services and support with Smart Help and Smart Remote Support

Product Information PDF

QuantStudio real-time PCR and digital PCR systems
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Real-time PCR solutions brochure
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QuantStudio 6 Pro Real-Time PCR Systems

QuantStudio 6 and 7 Pro Real-Time PCR Systems

The Applied Biosystems QuantStudio 6 and 7 Pro real-time PCR systems are designed to offer high quality and excellent reliability with features to deliver a smart workflow experience that brings lab-changing qPCR innovations to your research. These systems feature an interactive 12.1-in touchscreen interface, intuitive software, pre-optimized protocol templates, and access to your data anytime, anywhere with our Connect cloud-based platform. Additional smart features are included to enable a personalized experience that improves efficiency and helps streamline your workflow.

  • Smart features—hands-free functionality includes facial authentication login, voice commands, and RFID reader
  • Manual error reduction—compatible with TaqMan Array Plates with RFID
  • Interchangeable blocks—flexibility to change block formats between 96- (0.1 and 0.2 mL) and 384-well, and TaqMan Array Cards* via simply pushing a button or using a voice command
  • Connect—access, analyze, and share data anytime, anywhere with Connect, our cloud-based platform for real-time information access, secure data storage, and online results sharing
  • Remote access—monitor experiments and access results remotely using the Instrument Connect app
  • Small footprint—minimal bench space required: only 54.7 x 33.8 x 52.5 cm (H x W x D) and 38 kg (wt)
  • Multiplexing—5 or 6 optical channels (21 filter combinations for QuantStudio 7 Pro system)
  • Automation compatible—increase walkaway time and productivity by adding automatic loading of one or two QuantStudio 7 Pro systems with the Orbitor RS2 Microplate Mover
  • Results you can trust—10 logarithmic units of dynamic range to detect differences as small as 1.5-fold
  • Easy access to help—embedded video help from the instrument
  • Peace of mind—superior services and support with Smart Help and Smart Remote Support

Product Information PDF

QuantStudio real-time PCR and digital PCR systems
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Real-time PCR solutions brochure
Download

QuantStudio 6 Flex Real-Time PCR System

QuantStudio 6 Flex Real-Time PCR System

The Applied Biosystem QuantStudio 6 Flex Real-Time PCR System is ideal for laboratories with multiple applications or end users on a limited budget. Easily interchangeable thermal cycling block formats let you select the format that best suits your project without having to move attached peripherals or computers. Choose from standard or 96-well Fast and 384-well formats.

Key features

Minimize up-front capital investment
With one instrument, you can interchange between 96-well, 96-well Fast, and 384-well formats.

Skip the learning curve
The intuitive software, easy touch-screen setup, and effortless block changes are designed to let you get started right away.

Achieve sensitivity and performance
Detect as small as 1.5-fold changes in singleplex reactions and 10 logs dynamic range.

Upgrade when you need it
The QuantStudio 6 Flex system can be seamlessly upgraded to a QuantStudio 7 Flex system by a field service engineer to give you additional automation, throughput, and multiplexing capabilities.

Product Information PDF

QuantStudio real-time PCR and digital PCR systems
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Real-time PCR solutions brochure
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QuantStudio 3D Digital PCR System

QuantStudio 3D Digital PCR System

The QuantStudio 3D Digital PCR System leverages high-density nanofluidic chip technology for investigations that involve detecting rare events, analyzing small differences between two targets, or counting the exact number of targets in a sample. Due to the higher precision, sensitivity, and absolute nature of digital PCR, it is ideal for the following applications:

  • Qualification of molecular standards used in traditional real-time PCR experiments
  • Pathogen detection and load determination
  • Rare-target detection such as somatic mutation detection in oncology research
  • GMO detection and contamination assessment
  • Generation of references and standards
  • Copy number variation

Specifications

  • Time to read 1 sample –  ~30 sec   
  • PCR detection method – Endpoint       
  • Detection channels – FAM/SYBR Green, VIC, ROX           
  • Partitions – Chip reaction wells   
  • Samples per chip – 1     
  • Targets per chip – 2    
  • Chip capacity in thermal cycler – 24          
  • Reaction wells per sample – 20,000       
  • Loading volume – 14.5 μL    
  • Sealed workflow – Yes       
  • Dynamic range – 5 logarithmic units    
  • Precision at 95% confidence interval –  ±10%

Key benefits

Simple workflow

A streamlined workflow with minimal sample handling enables turnkey processing. Just load and go.

Affordable

Less than half the price of competing platforms, making new digital PCR technology within reach for most labs.

Absolute quantification

20,000 reaction wells yield data in copies/μL, enabling high precision and sensitivity without the need for a standard curve.

Sealed system

Helps limit contaminants with a sealed chip and no exposed sample transfer steps.

 Easily fits in populated spaces

Small footprint (7 x 5 x 9 in.) will fit most benchtops and can be moved when needed.

Compatible

Use your existing Applied Biosystems™ TaqMan® Real-Time PCR Assays for a digital result.

Product Information PDF

QuantStudio real-time PCR and digital PCR systems
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Real-time PCR solutions brochure
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Applied Biosystem QuantStudio 5 Real-Time PCR Systems

Applied Biosystem QuantStudio 3 and 5 Real-Time PCR Systems

When your data is always within reach, answers are never far away. The QuantStudio 3 and 5 Real-Time PCR Systems, combined with our Connect cloud-based platform, allows you to remotely monitor your runs, access results, and collaborate with colleagues anywhere, anytime.*

Obtain results you can trust—Detect differences in target quantity as small as 1.5-fold in singleplex reactions and obtain 10 logarithmic units of linear dynamic range

Helps save valuable time—Applied Biosystems™ VeriFlex™ Blocks with 3 or 6 independent temperature zones provide flexibility to run multiple experiments simultaneously. Fast thermal cycling is also available, enabling results in less than 30 minutes.

Real-time PCR application

  • Gene expression
  • miRNA profiling
  • SNP genotyping
  • Copy number variation
  • Protein thermal shift
  • High resolution melt
  • Pathogen detection

Product Information PDF

QuantStudio real-time PCR and digital PCR systems
Download
Real-time PCR solutions brochure
Download

Applied Biosystem QuantStudio 3 Real-Time PCR Systems

Applied Biosystem QuantStudio 3 and 5 Real-Time PCR Systems

When your data is always within reach, answers are never far away. The QuantStudio 3 and 5 Real-Time PCR Systems, combined with our Connect cloud-based platform, allows you to remotely monitor your runs, access results, and collaborate with colleagues anywhere, anytime.*

Obtain results you can trust—Detect differences in target quantity as small as 1.5-fold in singleplex reactions and obtain 10 logarithmic units of linear dynamic range

Helps save valuable time—Applied Biosystems™ VeriFlex™ Blocks with 3 or 6 independent temperature zones provide flexibility to run multiple experiments simultaneously. Fast thermal cycling is also available, enabling results in less than 30 minutes.

Real-time PCR application

  • Gene expression
  • miRNA profiling
  • SNP genotyping
  • Copy number variation
  • Protein thermal shift
  • High resolution melt
  • Pathogen detection

Product Information PDF

QuantStudio real-time PCR and digital PCR systems
Download
Real-time PCR solutions brochure
Download

7500 Fast & 7500 Real-Time PCR System

7500 Fast & 7500 Real-Time PCR System

Applied Biosystems™ 7500 Real-Time PCR Systems offer you high performance, multicolor real-time PCR wherever your research takes you. Choose from our Fast and standard 7500 systems.  Interested in a system for diagnostic research? We also offer the 7500 Fast Dx Instrument for your diagnostic needs.

  • Powerful, five-color platform is calibrated for the broadest range of dyes available: FAM™⁄SYBR® Green I, VIC®⁄JOE™, NED™⁄ TAMRA™⁄ Cy3™, ROX™⁄Texas Red®, and Cy5™ dyes
  • Specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets
  • Advanced multi-componenting algorithm minimizes spectral crosstalk – superior for multiplexing
  • User-friendly software includes plate set-up wizards, multi-plate data viewing capabilities, and advanced analysis tools to make data processing simple and straightforward
7500 Real-Time PCR System
  • 96 well format,
  • 5-color real-time PCR
  • 0.2ml block
  • 5-color dye flexibility in a trusted 96-well format
7500 Fast Real-Time PCR System
  • 96-well format, high-speed thermal cycling, delivering high-quality results in less than 30 minutes
  • 0.1ml block
  • Offers robust high-resolution melting analysis
7500 Fast Dx Real-Time PCR System
  • 96-well, 5-color real-time PCR for in vitro diagnostic use
  • 0.1ml block
  • Delivers performance required for high-quality results

Product Information PDF

QuantStudio real-time PCR and digital PCR systems
Download
Real-time PCR solutions brochure
Download