UltiMate 3000 series
Thermo Scientific UltiMate 3000 RSLCnano UHPLC System
The Thermo Scientific™ UltiMate™ 3000 RSLCnano modular system offers precision, productivity and
performance for all your low flow UHPLC needs
| Thermo Scientific UltiMate 3000 RSLCnano System | |
|---|---|
| Recommended flow range | 50 nL/min–50 μL/min (controlled with dedicated flow meters); 50–2500 μL/min with ternary micro pump (only in NCS-3500RS) |
| Pressure range | With nano ProFlow flow meter: 860 bar (12,500 psi) With capillary flow meter: 800 bar (11,600 psi) With micro flow meter: 800 bar (11,600 psi) |
| System delay volume | <350 nL in pre-concentration configuration |
| Retention time RSD | ≤0.2% RSD or < 0.1 SD whichever is greater with a 30 min gradient |
| Safety features | System wellness monitoring, leak sensors, active rear-seal wash system, excess pressure monitoring, emergency shut-down |
| pH range | 2–10 |
| Wetted parts | MP35N, titanium, PEEK, UHMW-PE, PTFE, FEP, sapphire, ZrO2, Al2O3, fused silica, SST, PCTFE, perfluoro elastomer (FFKM) |
| NCS-3500RS - Binary High Pressure Gradient (HPG) Pump with Ternary Micro Pump and Column Compartment NCP-3200RS - Binary HPG Pump |
|
| Binary High Pressure Gradient (HPG) Pump | |
| Flow rate range and maximum pressure | Nano ProFlow flow meter: 0–1,500 nL/min (recommended: 50–1,500 nL/min) 900 bar (13,050 psi) at full flow range Capillary flow meter: 0–15 μL/min (recommended: 0.5–10 μL/min) 800 bar (11,600 psi) at nominal flow rate 5 μL/min Micro flow meter: 0–50 μL/min (recommended: 5–50 μL/min) 800 bar (11,600 psi) at nominal flow rate 25 μL/min |
| Gradient delay volume | < 25 nL |
| Number of solvent channels | 2 |
| Wetted parts HPG | MP35N, titanium, PEEK, UHMW-PE, PTFE, FEP, ZrO2, Al2O3, fused silica, sapphire, PCTFE, SST |
| Ternary Micro Pump (Only in NCS-3500RS) | |
| Micro pump (low pressure gradient) | Flow rate range: 0–2500 μL/min; recommended 5–2500 μL/min; gradient formation is recommended from 50 μL/min |
| Maximum pressure | 620 bar (9,000 psi) |
| Number of solvent channels | 3 |
| Delay volume | 220 μL |
| Proportioning accuracy/precision | ±1.0% of full scale/<0.3% SD |
| Wetted parts | Titanium, PEEK, UHMW-PE, PTFE, FEP, ZrO2, Al2O3, perfluoro elastomer (FFKM) |
| Column Compartment (Only in NCS-3500RS) | |
| Temperature range | Room temperature + 7 ˚C up to 75 ˚C |
| Temperature accuracy/precision | ±0.5 ˚C (at 50 ˚C setpoint)/±0.1 ˚C |
| Temperature stability | ±0.1 ˚C (at 50 ˚C setpoint) |
| Heat-up time | From 35 ˚C to 65 ˚C in 12 min at an ambient temperature of 25 °C |
| Switching valves | Up to two valves 6-port 2-position (port-to-port volume: 91 nL, maximum pressure: 860 bar (12,500 psi)) 10-port, 2-position (port-to-port volume: 114 nL, maximum pressure: 860 bar (12,500 psi)) |
| Capacity | Maximum available width for column plus fittings: 350 mm; coiled fused silica columns |
| Features | Humidity sensor (column compartment), leak sensor, gas leak sensor, active rear-seal wash system, excess pressure monitoring |
| NCS-3500RS/NCP-3200RS Features and Dimensions | |
| GLP features | Column tracking |
| Dimensions | NCS-3500RS (h × w × d): 36 × 42 × 51 cm (14.2 × 16.5 × 20 in.) NCP-3200RS (h × w × d): 21 × 42 × 51 cm (8.3 × 16.5 × 20 in.) |
| Weight | NCS-3500RS: 32 kg (70.6 lb); NCP-3200RS: 17.5 kg (38.6 lb) |
| Power requirements (automatic voltage selection) | 100–120 V, 60 Hz 200–240 V, 50 Hz; max 300 VA |
| PC connection | USB 2.0; USB hub with three integrated sockets |
| I/O interfaces | Two digital inputs and two programmable outputs |
| Additional communication port | 15-pin D-Sub port for connection of a solvent rack or degasser |
| Autosampler (WPS-3000TPL RS) | |
| Injection volume range | 0–20 μL (recommended 20 nL–20 μL with different nanoViper sample loops); upgrade kit for injection volumes up to 125 μL available |
| Sample capacity | 3 × well plates (128 × 86 mm) 15 × 10 mL vials for reagents, diluents, and transport liquids* |
| Sample formats | 96 (deep) well plate, 384 (deep) well plate, sealed or open; 40 standard autosampler vials, 1.8 mL, sealed or open* |
| Injection cycle time | <30 s for a 1 μL full-loop injection |
| Maximum pressure | 860 bar (12,500 psi) |
| Injection methods | Full loop and partial loop injections, low-dispersion mode, μL pick-up, user-defined programs |
| Injection technique | Needle-in-needle with programmable needle wash |
| Injection precision | <0.4% RSD for 1 μL full loop injection |
| Injection linearity | Correlation coefficient > 0.9995, at 100 to 500 nL partial-loop injections, caffeine in water |
| Carryover | <0.02% for caffeine with external wash |
| Sample cooling | 4–45 ˚C, or 22 ˚C below ambient |
| Biocompatible version | Yes (upgrade kit available); |
| Fraction collection | Yes, with sample cooling (WPS-3000TFC, extra parts required: nano/cap modification kit and 6-port 2-position C72 valve) |
| Wetted parts | PEEK, SST, PAEK, PCTFE, PEEKsil™, fused silica |
| Dimensions (h × w × d) | 36 × 42 × 51 cm (14.2 × 16.5 × 20 in.) |
| Weight | 24 kg (53 lb) |
| Power requirements (automatic voltage selection) | 100–120 V, 60 Hz 200–240 V, 50 Hz |
| PC connection | USB; USB hub with three integrated sockets |
| I/O interfaces | Four digital inputs and four programmable outputs |
| UV/Vis Detector (VWD-3400RS) | |
| Data collection rate | Up to 200 Hz (in single wavelength mode); 5 Hz in multichannel mode |
| Maximum number of channels | 4 |
| Lamps | Deuterium lamp, Tungsten lamp Temperature control for both lamps |
| Drift | 4.0 mAU/h |
| Wavelength range | 190–900 nm ±1 nm |
| Noise | Typically < 0.05 mAU at 254 nm |
| Wavelength accuracy | 1 nm |
| Flow cell volume | 3 nL for nano LC; 45 nL for capillary LC; 180 nL for micro LC |
| Dimensions (h × w × d) | 16 × 42 × 51 cm (6.3 × 16.5 × 20 in.) |
| Weight | 15 kg (33 lb) |
| Power Requirements (automatic voltage selection) | 100–120 V, 60 Hz 200–240 V, 50 Hz |
| PC connection | USB |
| I/O and analog interfaces | Two digital inputs and two programmable outputs Two analog inputs available as an option via DAC plug-in module |
| Solvent Rack with Degasser (SRD-3400) | |
| Capacity | Sixteen 0.5 L reservoirs |
| Degassing channels | 4 (SRD-3400); 0 (SR-3000) |
| Channel volume | 670 μL |
| Wetted materials | Teflon AF, PEEK, and Tefzel |
| Status LEDs | Power, vacuum pump status, error (vacuum and/or leak) |
| Power supply and communication | 15-pin D-Sub (through UltiMate 3000 pumps) or external power supply |
| Dimensions (h × w × d) | 10 × 42 × 51 cm (3.9 × 16.5 × 20 in.) |
| Weight | With internal degasser: 4.8 kg (10.6 lb) |
| *Contact local sales representative for specific information | |
| Software | |
| Stand-alone UltiMate 3000 RSLCnano system | Thermo Scientific™ Chromeleon™ 7.2 SR4 or later, Chromeleon 6.8 SR16 or later* |
| UltiMate 3000 RSLCnano with Thermo Scientific MS detectors | Thermo Scientific Standard Instrument Integration (SII) 1.2 or later for Xcalibur™ and Chromeleon 7.2 SR4 or later; Chromeleon 7.2 SR4 or later* |
| UltiMate 3000 RSLCnano with Bruker MS detectors | DCMSLink™ based on Chromeleon 6.8 SR16 or later for Bruker Compass™/HyStar™* |
| *Contact local sales representative for more details about instrument control | |
