Protein Quantitation Guide
Highly referenced kits you can trust
Our ELISA kits have been referenced in over 3,000 publications and our multiplex assays cited in over 1,000 publications.
We offer a wide range of immunodetection-based products—in convenient, ready-to-use formats for sensitive, specific detection of intracellular or extracellular proteins. We have developed antibody pair kits, enzyme-linked immunosorbent assay (ELISA) kits for single-analyte analysis, and multiplex assays for multi-analyte analysis. Our kits undergo a rigorous validation process for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency, helping to enable dependably accurate results. You can use our Invitrogen™ and Thermo Scientific™ immunoassays to investigate any of these popular specific areas and more:
Table 4. Specifications for kit development.
| Specifications | Description |
|---|---|
| Benchmarking to ELISA | Correlates with ELISA data |
| Recovery | Tested on serum and plasma |
| Sensitivity* | Physiologically relevant levels, <10 pg/mL (based on detectable signal >2 SD above background) |
| Precision | Inter-assay CV: (between assays) Intra-assay CV: (within assays |
| Specificity | Cross-reactivity tests are performed with other analytes and antibodies |
| Linearity of dilution | High coefficient of correlation between sample dilutions and expected concentration over the range of the assay |
| Parallelism to natural samples | Recombinant standards are compared to natural samples to ensure equivalency |